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Isolation and Partial Purification of Extracellular Enzyme (1, 3)-β-D Glucanase from Trichoderma reesei (3929)

Author(s): R. Saravanan | V. Pavani Devi | A. Shanmugam | D. Sathish Kumar

Journal: Biotechnology
ISSN 1682-296X

Volume: 6;
Issue: 3;
Start page: 440;
Date: 2007;
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Keywords: Trichoderma reesei | 3 D glucanases | PDA medium | SDS-PAGE

The fungus Trichoderma reesei produces extracellular lytic enzymes such as β-1, 4 glucanases, β-1, 6 glucanases and β-1, 3 D glucanases. In the present study the PDA medium was used for the production of β-1, 3 glucanases from the fungal strain. Extra cellular protein (β-1, 3 glucanase) was partially purified by ammonium sulphate precipitation and dialysis. The amount of reducing sugar (126 mg/100 mL) liberated by the action of enzyme was determined by using DNS method. The molecular weight of the enzyme (52-62 kDa) was determined through SDS-PAGE.

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