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Isolation of some PUFA from edible oils by argentated silica gel chromatography

Author(s): Guil-Guerrero, J. L. | Campra-Madrid, P. | Navarro-Juárez, R.

Journal: Grasas y Aceites
ISSN 0017-3495

Volume: 54;
Issue: 2;
Start page: 116;
Date: 2003;
Original page

Keywords: Argentated silica gel column chromatography | Borage | Linseed | Polyunsaturated fatty acid | Shor tfin mako (Isurus oxyrinchus) | Sunflower | Ácidos grasos poliinsaturados | Borraja | Cromatografía en columna de gel de sílice impregnado con nitrato de plata | Girasol | Lino | Marrajo (Isurus oxyrinchus)

Four edible oils, linseed, borage and sunflower seed oils, and the liver oil of shortfin mako (Isurus oxyrinchus), have been fractionated by open column liquid chromatography, using partially argentated silica gel as stationary phase, looking for purify polyunsaturated fatty acids (PUFA). By this method, linoleic (LA, 18:2n6),α-linolenic (ALA, 18:3n3), γ-linolenic (GLA, 18:3n6), stearidonic (SDA, 18:4n3), eicosapentaenoic (EPA, 20:5n3) and docosahexaenoic (DHA, 22:6n3) fatty acid esters have been isolated from these oils with high purity percentages and yields.Los aceites comestibles de lino, girasol, borraja y el de hígado de marrajo (Isurus oxyrinchus), se fraccionaron mediante cromatografía líquida en columna abierta, usando como fase estacionaria gel de sílice parcialmente impregnado con nitrato de plata, con el objetivo de purificar varios ácidos grasos poliinsaturados (PUFA). Mediante este método, se aislaron los ésteres metílicos de los ácidos linoleico (LA, 18:2n6), α-linolénico (ALA, 18:3n3), γ- linolénico (GLA, 18:3n6), estearidónico (SDA, 18:4n3), eicosapentaenoico (EPA, 20:5n3) y docosahexaenoico (DHA, 22:6n3) con altas purezas y rendimientos.

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