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Isolation of subtelomeric DNA sequences labelling sheep and goat chromosome ends

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Author(s): Vaiman Daniel | Brunialti Ana | Bensaada Mohamed | Derbois Céline | Vaiman Anne | Crawford Allan | Metezeau Philippe | Cribiu Edmond

Journal: Genetics Selection Evolution
ISSN 0999-193X

Volume: 32;
Issue: 6;
Start page: 599;
Date: 2000;
Original page

Keywords: telomeres | FISH | sheep | goats

ABSTRACT
Abstract Two techniques that make it possible to isolate telomere DNA are presented, using sheep as an example. The first technique is based upon the screening of a sheep BAC library with PCR amplified DNA segments preserved from high-power laser beam irradiation. Twenty-three BACs hybridising to 13 subtelomeric regions in sheep and goats were obtained (out of 27 in the sheep complement), of which 13 recognised more than one region, telomeric or not. Twenty-three microsatellites were isolated from these BACs and 22 were genetically mapped on the sheep international genetic map, always consistently with the cytogenetical localisation in 17 cases out of 22. These results are discussed. The second technique is based upon the selective cloning of subtelomeric enriched DNA. Preliminary results were obtained by this approach.

Tango Jona
Tangokurs Rapperswil-Jona

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