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Kinetic of Expression of a Plantibody in (Nicotiana tabacum) Plants Cultivated in Different Substrates (Zeolite and Soil)

Author(s): Leonardo Gomez | Rodolfo Valdes | Merardo Pujol | Sigifredo Padilla | Tatiana Gonzalez | Milagros Font | Williams Ferro | Julio Sanchez | David Gavilan | Elio Espinosa | Yenisleydis Avila | Cristina Garcia | Otto Mendoza | Deborah Geada | Yordan Issac | Yarusenki Lescaille | Adelma Perez | Guirmaray Silva | Adrian Conde | Lorely Mila | Jose Brito

Journal: Journal of Agronomy
ISSN 1812-5379

Volume: 10;
Issue: 1;
Start page: 20;
Date: 2011;
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Keywords: plantibody | Hepatitis B surface antigen | transgenic plants | zeolite

This study reviewed the biomass production and expression of a plantibody in Nicotiana tabacum plants cultivated in zeolite, zeolite+soil and soil under confined conditions with the hope that results in the zeolite variant can be comparable with results in soil (best case). Production of leaves (168.4-228.8 g plants-1) and stems (148.0-190.4 g plants-1) achieved the highest values at the 8th week of cultivation. Non-significant differences were detected among substrates for production of leaves (p = 0.0920); meanwhile production of stems showed statistical significances between zeolite (148.0 g plants-1) and the mixture of zeolite and soil (190.4 g plant-1, p = 0.0422). The highest plantibody concentration in leaves ranged 56.7-71.5 µg mL-1 showing significant differences between zeolite and soil (p = 0.0219). The expression pattern of the plantibody was similar in all substrates reaching maximum values of plantibody amount (mg m-2) at 8th week. The expression level of plantibody ranged 0.19±0.05 (soil)-0.13±0.02% (zeolite). The optimal moment for purifying plantibody was at the 7th week when zeolite was used as substrates. Results of purification experiments performed to corroborate that plantibody produced by plants cultivated in zeolite was functional allow confirming a value of plantibody recovery higher than 40%, high level of plantibody purity and non-statistical differences in the antibody affinity constant. As concise outline, results obtained from this study provide information to conclude that although zeolite experiment results were lower than those observed in the mixture of zeolite and soil and soil, zeolite can be used as substrate for cultivation of transgenic tobacco plants employed in plantibody large-scale production under assessed conditions.
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