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Laccase Activity from Fresh Fruiting Bodies of Ganoderma sp. MK05: Purification and Remazol Brilliant Blue R Decolorization

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Author(s): Saranyu Khammuang | Rakrudee Sarnthima

Journal: Journal of Biological Sciences
ISSN 1727-3048

Volume: 9;
Issue: 1;
Start page: 83;
Date: 2009;
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Keywords: White rot fungi | ligninolytic enzyme | oxidoreductase | synthetic dye | anthraquinone dye | biotechnology

ABSTRACT
The primary aim of this study was to screen for laccase activity from various mushroom fruiting bodies found in Maha Sarakham and nearby provinces of Northeast of Thailand. The enzyme activity was followed by a spectroscopic property of an oxidized product of 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) at 420 nm. The results found that crude water extract of mushroom Ganoderma sp. MK05 gave the highest specific activity of laccase. Consequence aim was to partial purification of the crude water extract of the selected mushroom and to evaluate the dye decolorization activity. The crude enzyme was partial purified by ammonium sulfate fractionation in the range of 40-70% saturation and followed with DEAE-cellulose anion exchange column to 1.34 and 3.07 purification folds, respectively. Native gel electrophoresis result showed one protein band with laccase activity. The partial purified laccase from this mushroom showed a high efficient decolorization ability of anthraquinone dye, Remazol Brilliant Blue R with above 80% within 5 h at 30°C.

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