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A Large-Scale Preparation Method of High Purity C-Phycocyanin

Author(s): Wenjun Song | Cuijuan Zhao | Suying Wang

Journal: International Journal of Bioscience, Biochemistry and Bioinformatics
ISSN 2010-3638

Volume: 3;
Issue: 4;
Start page: 293;
Date: 2013;
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Keywords: Spirulina platensis | c-phycocyanin | purification | chromatography

In this paper, a process for large-scale purification of high purity C-phycocyanin (C-PC) has been set up. Spirulina platensis dried powder was incubated with 1 mg/ml lysozyme and then ruptured by using high pressure homogenizer. The crude extract was precipitated by 50% saturation ammonium sulfate, and purified by hydrophobic interaction chromatography using Phenyl Sepharose 6 FF, ion exchange chromatography using DEAE Sepharose FF and gel filtration chromatography using Sephacryl S-100 HR. The final recovery of C-PC was 42.03% with purity ratio (A620/A280) of 5.32.
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