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A Leishmania infantum FML-ELISA for the Detection of Symptomatic and

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Author(s): Behnam Mohammadi-Ghalehbin | Gholam Reza Hatam | Bahador Sarkari | Mehdi Mohebali | Zabih Zarei | Mansoureh Jaberipour | Shahab Bohlouli

Journal: Iranian Journal of Immunology
ISSN 1735-1383

Volume: 8;
Issue: 4;
Start page: 224;
Date: 2011;
Original page

Keywords: Canine | DAT | ELISA | Iran | PCR | Visceral Leishmaniasis

ABSTRACT
Background: Visceral leishmaniasis (VL) is caused by Leishmania infantum in Mediterraneanbasin and is an endemic disease in some parts of Iran. Canines are the mainreservoirs of VL in most of the endemic areas. Different serological methods have beenintroduced for diagnosis of canine visceral leishmaniasis (CVL). Objective: In this surveya Fucose-Mannose Ligand (FML) ELISA, using native L. infantum antigen, wasdeveloped and its validity for detection of infected dogs in comparison with direct agglutinationtest (DAT) and PCR was evaluated. Methods: Blood samples of sixty ownershipdogs (≤ 3 years old) were collected from Meshkin-shahr district in Ardabil province,North-west of Iran. Sera were separated for serological assays (DAT and FMLELISA)and the buffy coats were collected for molecular evaluation. Results: Two outof the 60 (3.33%) samples were found to be positive (antibody titer of ≥ 1/320) in DATwhile seven of the 60 (11.66%) samples were positive by FML-ELISA. Nine out of 60(15%) buffy coat samples showed a band about 680 bp indicative of L. infantum inPCR. Three out of 60 dogs had Kala-azar symptoms and were positive by PCR andFML-ELISA, while two of these three dogs had antibody titers >1/320 in their serumsamples. The sensitivity and specificity of FML-ELISA for the detection of CVL inboth symptomatic and asymptomatic dogs were found to be 77.8% and 100%, respectively.Conclusion: Considering the acceptable sensitivity and high specificity of FMLELISA,use of this serological method can be recommended for epidemiological surveysof CVL.

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