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Ligninolytic Enzymes of Lentinus polychrous Grown on Solid Substrates and its Application in Black Liquor Treatment

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Author(s): Wipawadee Budda | Rakrudee Sarnthima | Saranyu Khammuang | Nipa Milintawisamai | Sawait Naknil

Journal: Journal of Biological Sciences
ISSN 1727-3048

Volume: 12;
Issue: 1;
Start page: 25;
Date: 2012;
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Keywords: solid-state fermentation | carbohydrate degrading | ligninolytic enzymes | laccase | Lentinus polychrous | B-glucosidase

ABSTRACT
In nature, lignin can be degraded by ligninolytic enzymes from several of white rot fungi. Since, they can produce and secrete ligninolytic enzymes for lignin degradation and use as a carbon source. In this study, ligninolytic enzymes from Lentinus polychrous were investigated using CuSO4 at various concentrations (0-2.4 mol g-1 solid substrate) as laccase inducer. It was found that ligninolytic enzymes production by the fungus showed similar pattern. The main enzyme activities detected was laccase, following with manganese independent peroxidase and manganese dependence peroxidase, respectively. No lignin peroxidase activity was detected. Carbohydrate degrading enzymes, -glucosidase and cellulase activities have been found in a low quantity. Optimum conditions for catalysis were at pH 3-4 and temperature of 55C. The crude laccase showed a good stability in distilled water as well as in buffers pH 6-8. The highest stability was at pH 7 and 30C. The crude laccase of Lentinus polychrous was also evaluated for black liquor decolorization.

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