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Localisation of aphidicolin-induced break points in Holstein-Friesian cattle (Bos taurus) using RBG-banding

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Author(s): Rodriguez Viviana | Llambí Silvia | Postiglioni Alicia | Guevara Karina | Rincón Gonzalo | Fernández Gabriel | Mernies Beatriz | Arruga María

Journal: Genetics Selection Evolution
ISSN 0999-193X

Volume: 34;
Issue: 6;
Start page: 649;
Date: 2002;
Original page

Keywords: cattle | chromosome | fragile sites | aphidicolin

ABSTRACT
Abstract Fragile sites (FS) seem to play a role in genome instability and may be involved in karyotype evolution and chromosome aberrations. The majority of common fragile sites are induced by aphidicolin. Aphidicolin was used at two different concentrations (0.15 and 0.30 μM) to study the occurrence of FS in the cattle karyotype. In this paper, a map of aphidicolin induced break points and fragile sites in cattle chromosomes was constructed. The statistical analysis indicated that any band with three or more breaks was significantly damaged (P < 0.05). According to this result, 30 of the 72 different break points observed were scored as fragile sites. The Pearson correlation test showed a positive association between chromosome length and the number of fragile sites (r = 0.54). On the contrary, 21 FS were identified on negative R bands while 9 FS were located on positive R bands.

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