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Looking at microbial metabolism by high-resolution 2H-NMR spectroscopy

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Author(s): Victor P. Kutyshenko | Petr M. Beskaravayny | Maxim V. Molchanov | Svetlana I. Paskevich | Dmitry A. Prokhorov | Vladimir N. Uversky

Journal: PeerJ
ISSN 2167-8359

Volume: 1;
Start page: e101;
Date: 2013;
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Keywords: Microbe adaptation | Microbial metabolism | 2H-HMR spectroscopy | High resolution NMR | Heavy water

ABSTRACT
We analyzed the applicability of high-resolution 2H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondrion isolated from rat liver and from aqueous extracts of homogenates of rat liver and other organs and tissues in the presence of high D2O contents. Such analysis is possible due to the fast microbe adaptation to life in the heavy water. It is also shown that some enzymatic processes typical for the intact cells are preserved in the homogenized tissue preparations. The microbial and cellular metabolic processes can be differentiated via the strategic use of cell poisons and antibiotics.
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Tango Rapperswil
Tango Rapperswil