Author(s): Ljudmilla Borisjuk | Hardy Rolletschek | Johannes Fuchs | Gerd Melkus | Thomas Neuberger
Journal: Materials
ISSN 1996-1944
Volume: 4;
Issue: 8;
Start page: 1426;
Date: 2011;
Original page
Keywords: NMR | MRI | seed quality | crop seed | lipid imaging | sucrose allocation | seed aging | 13C
ABSTRACT
Low field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentration differed by >10 fold. Little use of high field NMR has been made in seed research to date, even though it potentially offers many opportunities for studying seed development, metabolism and storage. Here we demonstrate how 17.5T and 20T NMR can be applied to image seed structure, and analyse lipid and metabolite distribution. We suggest that further technical developments in NMR/MRI will facilitate significant advances in our understanding of seed biology.
Journal: Materials
ISSN 1996-1944
Volume: 4;
Issue: 8;
Start page: 1426;
Date: 2011;
Original page
Keywords: NMR | MRI | seed quality | crop seed | lipid imaging | sucrose allocation | seed aging | 13C
ABSTRACT
Low field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentration differed by >10 fold. Little use of high field NMR has been made in seed research to date, even though it potentially offers many opportunities for studying seed development, metabolism and storage. Here we demonstrate how 17.5T and 20T NMR can be applied to image seed structure, and analyse lipid and metabolite distribution. We suggest that further technical developments in NMR/MRI will facilitate significant advances in our understanding of seed biology.
