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Lymphocyte Cytotoxicity of oxLDL in Patients with Atherosclerosis

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Author(s): Mohammad Jafar Mahmoudi | Maryam Mahmoudi | Fereydoon Siassi | Fazel Shokri | Mohammad Reza Eshraghian | Amir Hassan Zarnani | Reza Chahardoli | Mona Hedayat | Jalal Khoshnoodi | Hashem Nayeri | Nima Rezaei | Ali-Akbar Saboor-Yaraghi

Journal: Iranian Journal of Immunology
ISSN 1735-1383

Volume: 8;
Issue: 1;
Start page: 27;
Date: 2011;
Original page

Keywords: Atherosclerosis | Cytotoxicity | oxLDL | Peripheral Blood Mononuclear Cells

ABSTRACT
Background: Atherosclerosis, a chronic inflammatory disease of the vessel wall is char-acterized by local and systemic immune responses to a variety of antigens. Oxidized low-density lipoprotein (oxLDL) is considered as an important determining factor in the pathogenesis of atherosclerosis. Objective: The purpose of this study was to investigate the degree of peripheral blood mononuclear cells (PBMC) vulnerability to in vitro oxLDL-induced cytotoxicity from atherosclerotic patients in comparison to healthy indi-viduals. Methods: Thirty patients with atherosclerotic lesions, confirmed by angiography, and 30 matched healthy individuals were investigated. PBMC was prepared from individuals' blood samples which were further stimulated with low dose (1 μg/mL) and high dose (50 μg/mL) of extensively oxidized LDL. MTT assay was utilized to measure cell viability and proliferation. Stimulation index (SI) was calculated as mean ratio of optical density (OD) of the stimulated cells divided by OD of untreated cells. Results: Low dose oxLDL treatment caused no significant proliferative or cytotoxic effect in the control group; however, similar treatment caused significant cytotoxic effect in the patient group compared to the controls (p=0.026). High dose oxLDL treatment induced more significant cytotoxicity in the patient compared to the control group (p=0.006). Comparison of the SI between the two groups of patients and controls showed significantly lower index by either the low (p=0.03) or the high dose (p

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