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MASS PRODUCTION OF THE BENEFICIAL NEMATODE STEINERNEMA CARPOCAPSAE UTILIZING A FED-BATCH CULTURING PROCESS

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Author(s): Devang Upadhyay | Rinu Kooliyottil | Sivanadane Mandjiny | Floyd L. Inman III | Leonard D. Holmes

Journal: ESci Journal of Plant Pathology
ISSN 2306-1650

Volume: 2;
Issue: 1;
Start page: 52;
Date: 2013;
Original page

Keywords: Steinernema carpocapsae | Xenorhabdus nematophila | fed-batch fermentation | beneficial nematodes

ABSTRACT
The present study deals with the batch and fed-batch mass production of Steinernema carpocapsae. S. carpocapsae is an entomoparasitic nematode that is used as a biological control agent of soil-borne crop insect pests. The ability and efficiency of fed-batch culture process was successful through the utilization of the nematode’s bacterial symbiont Xenorhabdus nematophila. Results from the fed-batch process were compared to those obtain from the standard batch process. The fed-batch process successively improved the mass production process of S. carpocapsae employing liquid medium technology. Within the first week of the fed-batch process (day six), the nematode density obtained was 202,000 nematodes mL−1; whereas on day six, batch culture mode resulted in a nematode density of 23,000 nematodes mL−1. The fed-batch process was superior to that of batch production with a yield approximately 8.8-fold higher. In fed-batch process, the nematode yield was improved 88.6 % higher within a short amount of time compared to the batch process. Fed-batch seems to make the process more efficient and possibly economically viable.
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