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METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF ESOMEPRAZOLE IN TABLET DOSAGE FORM BY RP-HPLC METHOD

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Author(s): Parthiban C | Divya swetha M | Pallavi V | Sowmya B | Jeevani | Prathyusha V

Journal: International Research Journal of Pharmacy
ISSN 2230-8407

Volume: 3;
Issue: 9;
Start page: 274;
Date: 2012;
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Keywords: Esomeprazole magnesium trihydrate | isocratic | RP-HPLC.

ABSTRACT
A simple, precise, rapid, and accurate RP-HPLC method has been developed to determine esomeprazole magnesium trihydrate using chromatographic separation isocratically on C18 column (5 μm, 150mm x 4.60mm) and acetonitrile: water (HPLC grade) in the ratio of 50:50 (v/v) as the mobile phase, at a flow rate of 1ml/min. Detection was carried out at 289 nm. The retention times was found to be 5.6, the method was linear in the concentration range of 10-60μg/ml with R2=0.997 respectively. The proposed method is successfully applied for the determination of drugs in commercial tablet preparation. The results of the analysis have been validated statistically and by recovery studies.
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