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The mice as biomodel in the genotoxicity assays, summary of final results of the study, two years of experiences, Finlay Institute, Cuba.

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Author(s): Daniel Francisco Arencibia Arrebola | Luis Alfredo Rosario Fernández

Journal: RETEL : Revista de Toxicología en Línea
ISSN 1668-091X

Volume: 27;
Start page: 1;
Date: 2010;
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Keywords: Spontaneous | induced | comet assay | micronucleis assay | chromosomal aberration assay | head sperm morphology assay | Balb-C mice | NMRI mice | OF-1 mice | C57/BL6/cenp mice | cyclophosphamide

ABSTRACT
For the new products evaluation is necessary to know the spontaneous and induced frequency of appearance of each one of the phenomenons that studies the genetic toxicology to use in the biomodel, for this way to realize that we are in front of a mutagenic substance and genotoxic, and this way to corroborate the existence or not an mutagenic effect and in that measured it is manifested, for this reason it is necessary the search of the ideal animal model. The aim of this editor letter is to give the final results of this study, when evaluating and to compare the spontaneous and induced indexes in mice of both sexes of the Balb-C, NMRI, OF-1 and C57/BL6/cenp lines, by means of these in vivo assays to determine the most efficient line, on the base of the significant appearance of lower spontaneous indexes and high induced indexes given by the sensibility to mutagenic substances as the cyclophosphamide. We was obtained as a result that the Balb-C line in both sexes differs significant with the other lines where they met the lower spontaneous indexes and the highest induced indexes with cyclophosphamide, keeping in mind the indicators such as epididymi spermatic concentration, spontaneous frequency of anomalous heads sperms, erythrocytes number in bone marrow with micronucleis, citotoxicity index (relationship among old erythrocytes/young erythrocytes), cells total with structural aberrations in the chromosomes, mitotic index (number of cells in metaphase) and the leukocytes percent in peripheral blood that they experience damage in the DNA according to level 1, 2, 3, 4 of smaller to more damage. Also the C57/BL6/cenp line was the less efficient and less sensitive to the mutagen, being obtained the higher spontaneous results and the lowest induced results.
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