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MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY

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Author(s): Nalan YILMAZ SARIÖZLÜ | Rasime DEMİREL | Merih KIVANÇ

Journal: Anadolu University Journal of Science and Technology - C. Life Sciences and Biotechnology
ISSN 2146-0264

Volume: 2;
Issue: 1;
Start page: 31;
Date: 2012;
Original page

Keywords: Hot spring | Eskişehir | BIOLOG | VITEK | Ribotype

ABSTRACT
In order to investigate and find out the bacterial community of hot spring waters in Eskişehir, Turkey, 7 hot spring water samples were collected from 7 different hot springs. All samples were inoculated using four different media (nutrient agar, water yeast extract agar, trypticase soy agar, starch casein agar). After incubation at 50 ºC for 14 days, all bacterial colonies were counted and purified. Gram reaction, catalase and oxidase properties of all isolates were determined and investigated by BIOLOG, VITEK and automated ribotyping system (RiboPrinter). The resistance of these bacteriawas examined against ampiciline, gentamisine, trimethoprime-sulphamethoxazole and tetracycline. As a result, heat resistant pathogenic microorganisms in addition to human normal flora were determined in hot spring waters (43-50 ºC) in investigated area. Ten different species belong to 6 genera were identified as Alysiella filiformis, Bordetella bronchiseptica, B. pertussis, Molexalla caprae, M. caviae, M. cuniculi, M. phenylpyruvica, Roseomonas fauriae, Delftia acidovorans and Pseudomonas taetrolens.
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