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Micropropagation of blackberry thornless cultivars

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Author(s): Alexandru Fira | Doina Clapa | Catita Plopa

Journal: Scientific Papers of the Research Institute for Fruit Growing Pitesti, Romania
ISSN 1584-2231

Volume: XXV;
Start page: 213;
Date: 2009;
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Keywords: Rubus | in vitro culture | 6-benzylaminopurine | acclimation | hydroculture.

ABSTRACT
The paper presents aspects regarding the in vitro propagation of the thornless blackberry cultivar ‘Thornless Evergreen’. For culture initiation, modified MS (Murashige & Skoog 1962) basal medium was used and, as growth regulator, 6-benzilaminopurine- 0,7mg/l (BAP). In order to establish the optimal variant of media regarding multiplication rate as well as the economic aspect, 3 variants of media were experimented, consisting of Murashige & Skoog 1962 (MS) salts, Myo inositol - 100 mg/l, Vitamin B1 - 1 mg/l, Vitamin B6 - 0.5 mg/l, Nicotinic Acid - 0.5 mg/l,36.7 mg/l FeNaEDTA and, as growth regulators, 6-benzilaminopurine in concentrations of 0.3, 0.5 and 0.7 mg/l. In order to optimize the multiplication phase, the influence of the number of inoculi/vessel upon multiplication rate was also tested for the blackberry. In vitro rooting of the shoots or shoot fragments originating from the multiplication phase was done on the rooting medium consisting of modified MS + 1 mg/l IBA. Ex-vitro rooting was also tested and acclimation in perlite, peat+perlite as well as acclimation in hydroculture were tested.
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