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Micropropagation of Chang Daeng (Rhynchostylis gigantea var. Sagarik) by embryogenic callus

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Author(s): Suphat Rittirat1 | Sompong Te-chato2* | Soisiri Kongruk2

Journal: Songklanakarin Journal of Science and Technology
ISSN 0125-3395

Volume: 33;
Issue: 6;
Start page: 659;
Date: 2011;
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Keywords: Chang Daeng | Rhynchostylis rubrum | micropropagation | embryogenic callus

ABSTRACT
Efficient propagation of Chang Daeng through embryogenic callus was investigated. Young shoot (approx. 10 mm)gave the highest percentage of multiple shoot formation (100), number of shoots (10 shoots/explant) and embryogenic callus(EC) formation (56%) Proliferation of the calli in both Vacin and Went (VW) and New Dogashima medium (NDM) supplementedwith 2% sucrose and 15% coconut water (CW) under light or dark condition was nearly the same. The color of calliin VW in both conditions was light yellow or cream while NDM provided bright yellow or yellowish green. Calli subculturedon NDM medium under light condition turned to green and then produced the highest percentage formation (79.8) andnumber of mature somatic embryos (9 embryos/callus). Within one month, those embryos germinated in the presence of ACin the culture medium. The shoots at length more than 3 cm with an average number of 2-3 roots were successfully transferredto soil. This empirical technique could be useful for micropropagation as well as genetic transformation in this plant.
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