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Micropropagation of GF 677 Rootstock

Author(s): Rahim Nazary Moghaddam Aghaye | Abbas Yadollahi

Journal: Journal of Agricultural Science
ISSN 1916-9752

Volume: 4;
Issue: 5;
Date: 2012;
Original page

The aim of this investigation was to study micropropagation of Almond GF rootstock on in vitro. Explants were cultured in MS medium containing 5 concentrations of 6- Benzylaminoporine (BAP) 0.1, 0.6, 1, 1.5 and 2 mg 1-1 along with control treatment. Each treatment includes 5 replications. After 5 weeks of culture, the results showed that the number and length of shoots in treatment containing 1 mg 1-1 BAP was more than the other treatments and control. The number of shoot that was obtained in treatment of 1 mg 1-1 BAP was 2.98 (Table 1). The lowest number of shoot was 0.4 due to control treatment. For rooting, explants with 3-5 cm length were transferred to different rooting treatments (Table 2). The results revealed that the best rooting was obtained with treatment 3 mg 1-1 IBA. Number and length of roots were 2.167 and 11.53, respectively. Also results showed that putting explants in dark for 1 week is more effective than light.
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