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Microwave-Assisted Rapid Extracellular Biosynthesis of Silver Nanoparticles Using Carom Seed (Trachyspermum copticum) Extract and in Vitro Studies

Author(s): Deshpande Raghunandan | Prashant Arunkumar Borgaonkar | Basawaraj Bendegumble | Mahesh Dhondojirao Bedre | Mantripragada Bhagawanraju | Manjunath Sooganna Yalagatti | Do Sung Huh | Venkataramana Abbaraju

Journal: American Journal of Analytical Chemistry
ISSN 2156-8251

Volume: 02;
Issue: 04;
Start page: 475;
Date: 2011;
Original page

Keywords: Microwave | Silver Nanoparticles | Biosynthesis | Carom Seed (Trachyspermum copticum) | Antibacterial Activity | Antioxidant Activity

Microwave-assisted rapid extracellular biosynthesis of silver nanoparticles was carried out by using carom seed (Trachyspermum copticum) extract as the reducing agent. The reaction mixture containing AgNO3 and carom seed extract when exposed to microwave irradiation resulted in reducing silver ions to bio-functionalized silver nanoparticles of size 6- 50 nm. The AgNP were characterized by UV-vis spectroscopy (UV-vis), X-ray diffraction (XRD), energy dispersive X-ray analysis (EDAX), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM). Themogravimetric analysis (TGA) and fourier transform infrared spectroscopy (FTIR) are used to understand the possible mechanism of biosynthesis. In this study, we have also investigated the antimicrobial and antioxidant activities of bio-functionalized AgNP. The antibacterial activity is investigated by measuring the zone of inhibition and antioxidant study is done using 1,1-diphenyl-2-picryl hydrazyl (DPPH) method.

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