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Modification of Spectroscopic Method for Determination of L-arginine in Different Tissue extracts

Author(s): M. A. A. Al-Bayati | F. J. I. Al-Shammaa | S. J. Ajeena

Journal: Pakistan Journal of Chemistry
ISSN 2220-2625

Volume: 2;
Issue: 1;
Start page: 1;
Date: 2011;
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Keywords: L-arginine | Modification | Spectroscopic Method

A simple technique was established for the specific modification of guanidine groups in different extracts of tissues involvingreaction with ninhydrin. The extent of the reaction is simply determined by spectrophotometric analysis. The method comparedwith HPLC. This modified method made easily determined in micro-quantities of L-arginine, which were operating conditions forthe successful determination of arginine were optimized. The results shed the light on reduction of interference substance with andgiven accuracy as compared with HPLC analytical method with acceptable recovery percent as well as this method given theamount of free type of arginine based on rapid reaction of amino group of L-arginine and compartmentalization was neglected thebound type profile. Finally the procedure was display high reproducibility and sensitivity of different biological tissues extracts,further studies need to explore the pharmacokinetic using of this method for given their applicability.
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