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Modulation of the captopril interference with the activity of some enzymatic biomolecules in monkey kidney vero cells by drug delivery mesoporous silica system

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Author(s): Roxana Popovici | Cosmin Mihai | E.M Seftel | Eveline Popovici | Pincu Rotinberg | Victor Voicu

Journal: Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
ISSN 1582-3571

Volume: 12;
Issue: 4;
Start page: 63;
Date: 2011;
Original page

ABSTRACT
The in vitro effect of different formulations of captopril on some cellular enzymatic equipments activities of monkey kidney Vero cells was investigated in the present research. The preparation of the samples of the mesoporous silica nanocomposites, loaded or not with captopril, was described and their effect on membranary Na+-K+-ATP-ase, cell Mg2+-ATP-ase, LDH, Px, GSH-Px, SOD, CAT, ACP, ALP activities were studied. The Vero cells were incubated, for a period of 144 hours, with growing medium renewed twice. When the cells reached confluence in the monolayer stage, the cultures were divided into control cell cultures and other 4 treated groups. To the 12 hours treated cells were added: Cap H2, SBA–15, unfunctionalized SBA-15_CapH2_RT and functionalized SBA-15_APTES_CapH2_80°C nanocomposites, each of them in a dose of 0.4μg./flask. As compared with the control Vero cells, which are characterized by a specific level of the enzymatic activities, the cultures treated with SBA-15 have not presented significant alterations of them. The comparative study of captopril interactions with some membrane bound and intracellular enzymatic biomolecules of monkey kidney Vero cells has revealed either an enhancement of membranary Na+-K+-ATP-ase, intracell total ATP- ase , LDH, ACP , and GSH-Px activities or a repression of cellular CAT, Px and SOD activities. These variations of the enzymatic activities – which induce modifications of the membranary and metabolic processes – could be due to a direct or indirect interaction of captopril with cellular (plasmalemma) or subcellular (organelles) structures and with intracellular biomolecules (enzymes, DNA, RNA etc.). The association of captoptil with SBA – 15 or SBA – 15 _ APTES mesoporous silica matrices and treatment of Vero cells with these nanocomposites were correlated with modulation of the captopril interference with the activity of investigated enzymatic biomolecules, its sense (stimulation or inhibition of enzymatic activity) and amplitude (high or small modulation ) being dependent of carrier type and peculiarities.
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