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Molecular Changes in Maspin and Bax Genes in Nitrosamine Induced Hepatocarcinogenesis

Author(s): Mohamed Hessien | Saad M. EL-Gendy; Hayat Ibrahim; Sayed Mahdy; H.F. Gomaa; Ahmed M. Rashad

Journal: Asian Journal of Medical Sciences
ISSN 2040-8765

Volume: 2;
Issue: 5;
Start page: 201;
Date: 2010;
Original page

Keywords: Box | beetroots | hepatocarcinogenesis | maspin | oxidative stress

This study was designed to evaluate the potential protective antioxidant and antihepatocarcinogenic effects of beetroots in rats adm inistered nitrosamine precursors (dibutylamine (DBA) and sodium nitrate). Also to monitor the changes in maspin and bax genes in parallel with the progression of nitrosamine induced oxidative stress. Rats were divided into four groups (n = 25 each). Group I was treated with DBA/NaNO3, group II was treated with DBA/NaNO3+ beetroots, in group III rats were treated with beetroots alone and group IV was control group. Markers of oxidative stress and the hepatocarcinogenesis marker A lpha-Feto Protein (AFP) were estimated in serum after 3, 6, 9 and 12 months. Simultaneously, the DNA levels of both cancer related genes maspin and Box were evaluated in liver. The data demonstrated that beetroots was able to antagonize the DBA/NaNO3-induced oxidative stress, where the levels of nitric oxide (NO), lipid peroxidation (LPO) and reduced glutathion (GSH) were improved in beetroots protected rats compared to the DBA/NaNO3- treated rats. This was accompanied with reduction in the level of AFP. The DNA content of maspin gene in precarcinogenated animals decreased in time dependant manner, whereas the level of bax DNA content was increased in parallel with the oxidative stress progression. No significant changes were observed in DNA content of both genes in beetroots-treated group and control group. Histologically, nitrosamine precursors showed distinct alterations such as loss of lobular architecture, fibrosis and the nuclei of many hepatocytes appeared malignant. The antioxidative/antihepatocarcinogenesis effect of beetroots was reflected on the histopathological architecture of liver and hepatocytes.
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