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MOLECULAR CHARACTERIZATION OF THE Bacillus thuringiensis (BERLINER) STRAINS 344 AND 1644, EFFICIENT AGAINST FALL ARMYWORM Spodoptera frugiperda (J. E. SMITH)

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Author(s): FERNANDO HERCOS VALICENTE | UBIRACI GOMES DE PAULA LANA

Journal: Revista Brasileira de Milho e Sorgo
ISSN 1676-689X

Volume: 7;
Issue: 3;
Start page: 195;
Date: 2008;
Original page

Keywords: insect pathology | Bacillus thuringiensis | cry genes

ABSTRACT
Bacillus thuringiensis (Berliner) strains 344 and 1644 belonging to the CNPMS/Embrapa (Maize and Sorghum National Research Center) Microorganism Bank, located in Sete Lagoas, MG, showed to be highly efficient in controlling fall armyworm, Spodotpera frugiperda (J.E. Smith). Strains 344 and 1644 were isolated from soil samples originated from Iguassu Falls and Rolândia, respectively, Paraná State, Brazil. While strain 344 harbors cry1Ab, cry1B, cry1E and cry1Fb genes, strain 1644 showed cry1B, cry1C, and cry1D and cry1Fb genes. The strain 344 crystal has bipyramidal shape and showed LC50 equal to 8.21 x 106 spores/mL. Strain 1644 has cuboid crystal and showed LC50 equal to 2.07 x 106 spores/mL. Comparative analysis of protein profile, plasmid and amplification with primers ERIC allowed the differentiation of the evaluated strains.
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