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MOLECULAR CHARACTERIZATION OF EXTENDED-SPECTRUM Β- LACTAMASES PRODUCING CTX-M-15 FROM GRAM NEGATIVE BACTERIA ISOLATED FROM PEDIATRIC AND NEONATAL INTENSIVE CARE UNIT

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Author(s): JADHAV S.V. | JADHAV R. | GANDHAM N. | MISRA R. | SINGH P. | UJAGARE M. | ANGADI K. | VYAWAHARE C.

Journal: International Journal of Drug Discovery
ISSN 0975-4423

Volume: 4;
Issue: 2;
Start page: 160;
Date: 2012;
Original page

Keywords: Extended-Spectrum β- Lactamases | CTX-M-15 | Pediatric intensive care unit | Neonatal intensive care unit

ABSTRACT
The rising rate of antimicrobial drug resistance in Enterobacteriaceae reduces the number of reliably effective drugs that can be used to treat infections. Gram negative bacteria producing β-lactamases that are resistant to many other antibiotics and very few antimicrobial agents remain effective as treatment option. Since the initial description of Extended Spectrum β-lactamases (ESBLs) production by Klebseilla pneumonia & Escherichia coli in the 1980s, strains of Enterobacteriaceae resistant to third-generation cephalosporins are increasingly being recognized globally. ESBL-producing Enterobacteriaceae strains have been frequently implicated in outbreaks in all intensive care units especially pediatric Intensive Care Unit (PICUs) and Neonatal Intensive Care Unit (NICU). Therefore, there have been many recent calls to intensify current infection control efforts aimed at reducing the emergence and dissemination of infections caused by antibiotic-resistant bacteria. The widespread use of Cefriaxone and/or cefotaximine has been proposed as a reason for the emergence of CTX-M enzymes. The increased frequency of isolation & reporting of CTX-M ESBLs is alarming and is likely to represent only the tip of iceberg for the underdeveloped continents where molecular technology for the analysis of ESBL enzymes is scares. To further delineate the mode of successful dissemination of ESBLs CTX-M-15 and to gain insights into the mechanism underlying this phenomenon we designed this study to assesses clonality & diversity of Enterobacteriaceae strains isolated from PICUs and NICUs of our hospital. Total of 100 blood samples were received from PICU and NICU during the study period of two months. 10 ESBLs were isolated from total 55 Gram negative bacteria, of that five were possessing CTX-M-15 by PCR methods.
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Tangokurs Rapperswil-Jona