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Molecular Cloning, mRNA Expression and Characterization of a Novel FAIM1 Gene from Chinese Banna Mini-Pig Inbred Line (BMI)

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Author(s): Jinlong Huo | Pei Wang | Yue Zhao | Hailong Huo | Lixian Liu | Rui Wang | Heng Xiao | Yangzhi Zeng

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 11;
Issue: 8;
Start page: 1080;
Date: 2012;
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Keywords: Banna Mini-pig Inbred line (BMI) | pig | FAIM1 | tissue expression | bioinformatics analysis | China

ABSTRACT
The complete CDS sequence of Banna Mini-pig Inbred line (BMI) gene FAIM1 was amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to Accession No.: JF271685. Sequence analysis revealed that the BMI FAIM1 encodes a protein of 200 amino acids that has high homology with the Fas apoptosis inhibitory molecule 1 proteins of five other species-cattle 95%, horse 95%, human 94%, mouse 92% and rat 91%. The phylogenetic tree analysis revealed BMI FAIM1 has a closer genetic relationship with the bovine FAIM1 than with those of horse, human, mouse and rat. Analysis by RT-PCR showed that BMI FAIM1 gene was over-expressed in lymph node, diencephalon, heart, muscle, moderately expressed in midbrain, spleen, lung, small intestine, fat and almost not expressed in other 9 tissues. Several microRNA target sites were predicted in the CDS of BMI FAIM1 mRNA for further studying this gene in the future.
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