Academic Journals Database
Disseminating quality controlled scientific knowledge

Molecular Identification by 16S rDNA Sequence of a Novel Bacterium Capable of Degrading Trichloroethylene

Author(s): Srijata Mitra | Pranab Roy

Journal: Journal of Biological Sciences
ISSN 1727-3048

Volume: 10;
Issue: 7;
Start page: 637;
Date: 2010;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: phylogenetics | 16S rRNA gene | trichloroethylene | strain 2479 | cereus

Trichloroethylene (TCE) is a widely used organic solvent and metal degreasing agent, one of the most frequently detected groundwater contaminants and a potential health hazard. A novel gram positive, rod shaped bacterial strain 2479 was isolated from soil near the oil depot site at Rajbandh, West Bengal (India) and its exact taxonomic position was investigated on the basis of 16S rRNA gene sequencing and Fatty acid methyl ester analysis (FAME). This novel strain was capable of degrading Trichloroethylene (TCE) efficiently. The major fatty acids detected in the strain 2479 were iso C15::0 (24.49%) and iso C16:0 (12.12%). The 16S rRNA gene of strain 2479 was amplified by using Bacillus specific primers and obtained 1465bp amplified product. Comparison of 16S rDNA region (1465bp) of the isolate 2479 by Ribosomal Database Project II – Sequence match showed greatest similarity with genus Bacillus sp. JDM-2-1(Accession No. EF584539). Phylogenetic analysis, involved the identification of homologous sequences, their multiple alignment, phylogenetic reconstruction and the graphical representation of the inferred tree was done in Phylogeny fr. package. Phylogenetic tree showed strain 2479 had 100% similarity with Bacillus cereus group. On the basis of phylogenetic data and Fatty Acid Methyl Ester Analysis, strain 2479 should be placed within the genus Bacillus and species cereus. This is the first instance, Bacillus cereus group being used in biodegradation of Trichloroethylene.
Affiliate Program      Why do you need a reservation system?