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Molecular Identification of Hog Cholera Viruses from Southern China

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Author(s): Wu Hongzhuan | Yee-wai Chan | Kit-man Wong | Frederick C. Leung

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 4;
Issue: 5;
Start page: 497;
Date: 2005;
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Keywords: Hog cholera virus (HCV) | RT-PCR | Sequencing

ABSTRACT
17 HCV infected tissue samples collected from Southern China between 1996 and 2000 were identified with a RT-PCR and sequencing, PCR primers used in this study were designed according to the sequence of E2 gene published in GeneBank.15 out of them were positive when detected by a RT-PCR, all the samples were parallel to recover virus in vitro, 5 were positive and two isolates were confirmed to be virulent with rabbit inoculation test. Sequence analysis revealed that HCV isolates from Southern China collected from different years homologous to different strains reported in GeneBank, among them, strain 529 similar to a Italian field strain PR/98/dp, 829 and HCV lapinized strain at the same group, 1026 was similar to Alfort strain, isolates 580and 856 at the same group, whereas GS-YZ isolates, a virulent strain from mainland China was at a separate group.
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