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Molecular markers for genetic stability of intergeneric hybrids Fragaria x Potentilla derived from tissue culture

Author(s): Anca Nicoleta SUTAN | Aurel POPESCU | Raluca GHEORGHE | Carmen Florentina POPESCU | Valentina ISAC

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XVI;
Issue: 2;
Start page: 146;
Date: 2009;
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Keywords: tissue culture | intergeneric hybrids | RAPDs | clonal fidelity

The effect of growth regulators, explant source and culture age on genetic stability of plants obtained from tissue culture propagation of ornamental strawberry “Serenata” were examined. Genomic DNAs of in vitro-derived shoots and control plant were extracted and compared by RAPD-PCR analyses. Ten primers (from 48 previously tested) were selected and used in RAPD analysis to prove the clonal fidelity (i.e. genetic stability) of the tissue culture-derived ornamental strawberry plants. The lack of polymorphisms in micropropagated plants screened through molecular markers was used to suggest genetic fidelity. Identicaly banding patterns of the RAPD profiles obtaining from vitroplants, regenerated via organogenesis or meristems culture, suggested that in the ornamental strawberry, variety “Serenata”, neither explant source, nor callus age or limited number of subcultures, in basal media supplemented with low concentration of growth regulators, were associated with occurence of somaclonal variation.
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