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Molecular Typing and Probiotic Attributes of a New Strain of Bacillus coagulans – Unique IS-2: a Potential Biotherapeutic Agent

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Author(s): M Ratna Sudha

Journal: Genetic Engineering and Biotechnology Journal
ISSN 2150-3516

Volume: 2010;
Start page: GEBJ-7;
Date: 2010;
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Keywords: Bacillus coagulans “Unique IS-2” | Molecular Identification | RAPD | Probiotics | Phylogeny

ABSTRACT
The genus Bacillus has been in use in the biotechnology industry for a very long time with a number of new cultures exhibiting avariety of benefits to humans. The present study focuses on phenotypic and genotypic characterization of a probiotic lactic acidbacteria, Bacillus coagulans (IS-2 a Unique-Biotech strain) isolated from human feces and contaminated soils. Probiotics are livemicrobes which when administered in adequate amounts confer a health benefit on the host and are increasingly used innutraceuticals, functional foods or in microbial interference treatment. In addition to morphological and biochemicalcharacterization, of this isolate, molecular characterization was done. In vitro assays performed to confirm the probiotic attributes ofthis isolate B.coagulans-Unique IS-2. The mole % G+C content of IS-2 strain was found to be 46 indicating the identity of the cultureas Bacillus coagulans. PCR based amplification of the 16S rDNA, using universal primers, and sequencing done to study the diversity,as it allows identification of prokaryotes as well as the phylogenetic placement of the isolates. 16S rDNA sequence data was used toidentify our lab isolates followed by RAPD profile of the new isolate- IS-2 of B. coagulans. The phylogenetic tree using Clustal Wconstructed by UPGMA based on the 16S rRNA gene sequence further confirmed that Unique Biotech strain IS-2 formed a coherentcluster with two other strains of B. coagulans namely B. coagulans ATCC 7050 and Bacillus coagulans NCIM 2030. Evaluation of itsprobiotic attributes revealed that Bacillus coagulans Unique IS-2 had promising Probiotic properties with reference to its survival ingastrointestinal conditions in the in-vitro assays and therefore offers excellent opportunities for its successful commercialization asa biotherapeutic agent.
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