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Morphological characteristics of free radical processes in endotoxin damage of liver

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Author(s): Babanin A.A. | Zacharova A.N. | Semyonova T.V. | Nesterov E.N. | Kaliberdenko V.B.

Journal: Морфологія
ISSN 1997-9665

Volume: 3;
Issue: 2;
Start page: 5;
Date: 2009;
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Keywords: endotoxin | liver | oxidative modification of proteins | peroxidation of lipids | peptides of average mas

ABSTRACT
One of the most important problems of the modern medicine is the damage of liver due to alcoholic disease. Our studies showed some features of morphological changes of liver tissues and level of biochemical markers of oxidative stress, due to experimental endotoxin intoxication. Investigations were done with the aim of modeling the endotoxin damage to the liver of rats. Investigation was carried out on 40 nonlinear rats, divided into 2 groups with 20 rats in each group. The animals of the first group were given for 7 days with intraperitoneal injections of Escherichia coli endotoxin (Sigma USA DO-235), dosage 0,1 mg/kg of body weight. Animals of the second group were used as a control. Character and level of liver damage was studied with the help of morphological methods, including electron and light microscopy. In studying of oxidative stress biochemical methods were used to define conjugated diens and dienketones, spontaneous oxidative modifications of proteins. The following results were revealed: 1) level and depth of morphological changes under endotoxin intoxication correlated with dynamic of content of products of lipid (conjugated diens and dienketones, malonic aldehyde) and protein. The tendency was shown to more significant increase of oxidative modification of proteins in blood serum. It corroborated the data about primary damage of proteins under the effect of free radicals. 2) Under the influence of endotoxins of intestinal microflora, severe discirculatory changes, fat and hydropic dystrophy of hepatocytes with symptoms of toxic damage of their nucleus were found, meanwhile increased functional activity of sinusoidal cells was observed. These changes happened due to direct effects of the endotoxin, and also as a result of oxidative stress. Powerful inductor of these changes was endotoxin. Obtained results showed, that endotoxin is the main factor, which leads to worsening of vital activity of the liver.
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