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Morphometric and immunohistochemical patterns of duodenal CCK-cells in rats with portacaval shunts

Author(s): Radosavljević Tatjana | Todorović Vera N. | Vučević Danijela | Šikić Branka

Journal: Medicinski Pregled
ISSN 0025-8105

Volume: 59;
Issue: 3-4;
Start page: 124;
Date: 2006;
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Keywords: rats | cholecystokinin | intestine | small | immunohistochemistry | portacaval shunt | surgical | liver cirrhosis | experimental

Introduction. End-to-side portacaval shunt (PCS) performed in rats is an appropriate experimental model of chronic liver insufficiency. Functional hepatic injury followed by numerous metabolic and endocrine disorders develops, as well. Due to impaired functional hepatic activity in PCS and to the well known role of the liver in CCK metabolism and elimination, delayed CCK blood­stream elimination may be responsible for increased CCK-A receptor activity. Therefore, we investigated immunohistochemical and morphometric features of duodenal CCK-cells in rats with PCS. Material and methods. Male Wistar rats (180-250g body weight) were used in this experiment. The animals were divided into two groups: PCS-animals (experimental group (n=27) which undenvent an operation) and C-unoperated animals [control group (n=11)]. Surgical technique End-to-side portacaval anastomosis was created using the technique of Lee and Fisher, modified by Bismuth el al. Eight weeks after the operation, animals were sacrificed. Pancreatic and hepatic weights were measured and samples taken from appropriate segments of the small intestine were used for morphometric and immunohistochemical CCK-cells determination. Immunohistochemical technique Peroxidase-antiperoxidase (PAP) technique described by Stenberger was applied for detection of intestinal CCK-immunoreactive cells. Results. Our results indicate that eight weeks after operation liver weight was reduced in animals with PCS compared to control animals (p
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