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Multi-Level Characterization of Protein Glycosylation

Author(s): Serenus Hua | Myung Jin Oh | Hyun Joo An

Journal: Mass Spectrometry Letters
ISSN 2233-4203

Volume: 4;
Issue: 1;
Start page: 10;
Date: 2013;

Keywords: Mass spectrometry | Glycosylation | Glycomics | Glycoproteomics

Recent developments in MS-based glycomics and glycoproteomics have rapidly advanced the field and pushed the boundaries of glyco-analysis into new territories. This review will lay out current workflows and strategies for characterization of the glycoproteome, including (in order of increasing complexity and information content) preliminary site mapping, compositional glycan profiling, isomer-specific glycan profiling, glycosite-specific glycopeptide profiling, and finally, glycoproteomic profiling.
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