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Mycobacterium Group: Identification and Sensitivities of Clinical Isolates Against First Line Drugs. I

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Author(s): Tanveer Khanum | Aliya Hayat | Ajaz Rasool | Saleem Hafiz | Rafia Azmat | Rukhsana Talat

Journal: Pakistan Journal of Biological Sciences
ISSN 1028-8880

Volume: 8;
Issue: 12;
Start page: 1794;
Date: 2005;
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Keywords: Mycobacterium sp. | | first line drugs sensitivity | staphylococcin

ABSTRACT
Mycobacterium infections play a very important role in the community especially in areas where the disease is endemic. Mycobacterial infections need to be treated with a mixture of agents to improve efficacy, to prevent resistant, or to overcome intrinsic resistant. In order to see the resistant pattern of the local isolates a prospective study was planned. About, 100 clinical isolates of mycobacterium were collected from local clinical laboratories they were confirmed by standard tests recommended by ASM manual and sensitivities were set up by resistant ratio method against the first line drugs, i.e., Streptomycin, Isoniazid, Ethambutanol Rifampicin. and Bac 201 was also found to have antimycobacterial activity. Over all 38% were Mycobacterium tuberculosis, 22% Mycobacterium xenopi, 16% Mycobacterium kansasai and 12% Mycobacterium thermoresistible 4% Mycobacterium merinum, 4% Mycobacterium fortutum and 4% Mycobacterium terraecomplex 70% were found to be slow grower, while 28% were fast grower and 2% were found to be moderate grower 22% were Photochromogen and 12% were Scotochromogen, 64% were non chromogenic. About 62% were atypical mycobacteria. Resistance pattern in this study was not very different from other studies. In this study highest resistance was to Rifampicin 30% followed by Isoniazid and Strptomycin 8%. Ethambutanol and Pyrazinamide were found to have relatively lower resistance 6 and 4% respectively. A protein antibiotic i.e., Staphylococcin Bac 201 was also tried which gave some interesting results, its known conc. i.e., 1218 AU mL-1. Sixty two percent were completely resistant to all 6 concs and 38% showed MIC as 1218 AU mL-1 , 25-64% resistant to first line drug has been observed. With the emergence of drug resistant mycobacteria it has become essential to carry out the drug susceptibility testing regularly.
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