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NaCl stress-induced growth, water and ions contents changes on in vitro selection of salt tolerant and salt sensitive callus of wheat (Triticum durum Desf.)

Author(s): Ayolié Koutoua 1,2 , El Yacoubi Houda 2 , Atmane Rochdi 2 , Kouakou Kouakou Laurent 1 , Kouakou Tanoh Hilaire 1*

Journal: International Journal of Biosciences
ISSN 2220-6655

Volume: 1;
Issue: 4;
Start page: 12;
Date: 2011;
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Keywords: : Callus | In vitro selection | salinity | salt tolerance | wheat.

Callus cultures tolerant to NaCl were developed from eight wheat genotypes using in vitro selection techniques. The accumulation of inorganic (Na+, Cl-and K+) solutes, water content and relative fresh weight were determined in selected (tolerant and sensitive) calli after a NaCl shock in order to evaluate their implication in salt tolerance of the selected lines. No growth reduction was observed in salt-tolerant calli compared to control while a significant (P

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