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Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol

Author(s): Nikita O. Ronzhin | Alexey V. Baron | Ekaterina S. Mamaeva | Alexey P. Puzyr | Vladimir S. Bondar

Journal: Journal of Biomaterials and Nanobiotechnology
ISSN 2158-7027

Volume: 04;
Issue: 03;
Start page: 242;
Date: 2013;
Original page

Keywords: Nanodiamond | Test-System | Glucose | Cholesterol

Model test-systems for biochemical determination of glucose and cholesterol are designed by covalent coupling of enzymes to the surface of modified nanodiamonds (MND). In both cases after immobilization on MND the enzymes exhibit activity and catalyze the formation of a colored product in the chain of successive enzymatic reactions of oxidative azocoupling (Н2О2—4-aminoantipyrine—phenol). Produced MND-enzymes complexes: function in DI water and various buffers, over a wide range of temperature and pH, provide for the linear product yield over a broad concentration range of analyzed substances, make possible to test cholesterol and glucose in vitro many times, exhibit functional activity after long storage at + 4°C.
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