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Nephrotoxicity study of Aristolochia fangchi in rats by metabonomics

Author(s): Qi LIANG | Ming XIE

Journal: Zhong Xi Yi Jie He Xue Bao
ISSN 1672-1977

Volume: 7;
Issue: 8;
Start page: 746;
Date: 2009;
Original page

Keywords: Aristolochia fangchi | nephrotoxicity | metabonomics | nuclear magnetic resonance | pattern recognition | rats

Objective: To study the changes of metabolites in rat urine after treatment of Aristolochia fangchi decoction by metabonomic method. Methods: Sixty-four male SD rats were divided into Aristolochia fangchi group and normal control group. Rats in the Aristolochia fangchi group were orally administered with 8.1 g/(kg·d) of Aristolochia fangchi and the normal control group was administered with equal volume of distilled water for 4 weeks. Twenty-four hour urine was collected at different time points (before, after 2- and 4-week administration and 2 weeks after administration) and their 1H nuclear magnetic resonance (NMR) spectra were acquired and subjected to data process, including principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) or orthogonal signal correction (OSC). The contents of blood urea nitrogen (BUN) and serum creatinine (SCr) and histopathological changes of the renal tissues were also detected. Results: The content of BUN of the Aristolochia fangchi group was markedly higher than that of the normal control group after 2-week administration (P
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