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Neuroimmune consequences of teratological insult induced by cyclophosphamide exposure during intrauterine life in mice

Author(s): Prakash | Singh G | Singh S

Journal: Neuroanatomy
ISSN 1303-1783

Volume: 6;
Issue: 1;
Start page: 6;
Date: 2007;
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Keywords: cyclophosphamide | neuroimmunity | mice | correlation | teratology

pups produced after cyclophosphamide exposure during intrauterine life. Cyclophosphamide in single doseof 10 or 20 mg/kg body weight was administered to pregnant mice on day 11 of gestation. Fetuses weredissected out on day 19 and studied for gross malformations. Brains and thymuses were collected for furtherinvestigations. Cyclophosphamide treated brains and thymuses were smaller in size and distorted in shape ascompared to control brains and thymuses. Protein estimation of brains revealed decline in both quantitativeand qualitative aspect. Study of brain cells and thymocytes revealed 1) increase in apoptotic cell count and2) increase in percent DNA fragmentation. In vitro cell proliferation assay exhibited decrease in both braincells and thymocytes. Similar pattern of response shown by brain cells and thymocytes conclude interactionbetween central nervous system and immune system insults produced by cyclophosphamide. Loss of certainneuropeptides has culminated in thymic compromise besides direct actions.

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