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The neutralization effect of methanol extract of Andrographis paniculata on Indian cobra Naja naja snake venom

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Author(s): Kadiyala Gopi, Kadali Renu, Mohan Raj, Dinesh Kumar and Muthuvelan B*

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 4;
Start page: 1010;
Date: 2011;
Original page

Keywords: Andrographis paniculata | Naja naja | neutralization.

ABSTRACT
The present study reports the antivenom properties of Indian medicinal plant Andrographis paniculata (Acanthaceae), commonly referred as ‘’Nilavembu’’ to reduce the effect of snake venomof Indian cobra Naja naja. Shade dried stem and leaf parts of the Andrographis paniculata were extracted with different solvents on the basis of polarity nature such as petroleum ether (polarity 0), ethyl acetate (4.4), methanol (5.1), and water (9.0). All the four extracts were tested for their antivenom activities through in vivo experiments. Among those, methanol extract of Andrographis paniculata has shown significant inhibition on neurotoxic symptoms caused by the venom (450 μg/ kg b.w) and prolonged the survival time of mice (22 ± 2 g) maximum up to 14.44 ± 0.55 h compared to other extracts. This in vivo screened active methanol extract was further tested for direct inhibitory activity on Naja naja snake venom major enzymes like; acetyl cholinesterase, hyaluronidase, ATPase, protease, and hemolytic activities in vitro. In these experiments, the venom was preincubated with different concentrations of Andrographis paniculata methanol extract at 37 °C for 1h before adding to the reaction mixture in vitro. The results confirmed that, the methanol extract of Andrographis paniculata possesses potent snake venom inhibitors. Phytochemical screening for active components of methanol extracts are still under investigation.

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