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A new method for quantifying endoglucanase activity based on Congo red-carboxymethyl cellulosecomplex depolymerisation

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Author(s): Martha Lucia Ortiz Moreno | Daniel Uribe Vélez

Journal: Orinoquia
ISSN 0121-3709

Volume: 15;
Issue: 1;
Start page: 7;
Date: 2011;
Original page

Keywords: Endoglucanase | carboxymethylcellulose | Congo red | cellulolytic fungus.

ABSTRACT
The aim of this work was to establish a method for quantifying endoglucanase activity based on the depolymerisation of the Congo red-polysaccharide complex. The mathematical viscosity and absorbancepatternsforCongo red-carboxymethylcellulose(CMC) culture media were thus described. The inoculated media'sviscosity and absorbance were monitored for 8 daysto measure the impact of a cellulolytic fungussuch as Trichodermaviride on the media. Enzymeconcentration was calculated by correlating reducing sugars'absorbance test results using3,5-dinitrosalicylic acid (DNS) with loss of CMC culture mediaabsorbance.Four cellulolytic deuteromycetesstrains were testedto assess the technique'sapplicability;it was found that usingCMC media absorbancelosswas effective for differentiatingstrains'endoglucanase activity. The Congored-CMC method was faster and more sensitive than the DNS method. This new method should thusbe appliedin screening assays when bioprospecting cellulolytic fungi in soil.
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