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Non-viral eNOS gene delivery and transfection with stents for the treatment of restenosis

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Author(s): Brito Luis | Chandrasekhar Saradha | Little Steven | Amiji Mansoor

Journal: BioMedical Engineering OnLine
ISSN 1475-925X

Volume: 9;
Issue: 1;
Start page: 56;
Date: 2010;
Original page

ABSTRACT
Abstract Background In this study, we have examined local non-viral gene delivery, transfection, and therapeutic efficacy of endothelial nitric oxide synthase (eNOS) encoding plasmid DNA administered using coated stents in a rabbit iliac artery restenosis model. Methods Lipopolyplexes (LPPs) with eNOS expressing plasmid DNA were immobilized on stainless steel stents using poly(D,L-lactide-co-glycolide) (PLGA) and type B gelatin coatings. The gene-eluting stents were implanted bilaterally in the denuded iliac arteries and eNOS transfection and therapeutic efficacy were examined 14 days after implantation. Results The results show that non-viral lipopolyplex-coated stents can efficiently tranfect eNOS locally in the arterial lumen assessed by PCR and ELISA. Human eNOS ELISA levels were significantly raised 24 hours after transfection compared to controls (125 pg eNOS compared to
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Tangokurs Rapperswil-Jona