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Novel bioactive actinomycetes from a niche biotope, Loktak Lake, in Manipur, India

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Author(s): Suchitra Sanasam, Salam Nimaichand, Debananda Ningthoujam

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 6;
Start page: 1707;
Date: 2011;
Original page

Keywords: Microbial diversity | Actinomycete | Loktak Lake | Sediments | Streptomyces.

ABSTRACT
Falling in the Indo-Burma biodiversity hotspot, Manipur is a promising area for microbial diversity studies. As a part of our ongoing research on actinomycete diversity of Manipur, sediments from various depths of the Loktak Lake – the largest freshwater lake in NE India - were sampled for isolation, bioactivity screening and characterization of the potent bioactive actinomycete isolates. We have obtained a total of 207 actinomycete isolates from the sediment samples of Loktak Lake. Based on the results of primary screening, 23 isolates were selected for secondary screening. Nine (9) strains showed significant antibacterial or broad spectrum antimicrobial (antibacterial and antifungal) activities. Phylogenetic analysisindicated that a majority of them were Streptomyces and nocardioform actinomycetes. Five lake isolates (LS1-81, LS1-88, LS1-117, LS1-128, and LS1-10C) were characterized as Streptomyces spp, 3 (LS1-145, LSCH-7, LSCH-31) belong to Pseudonocardia and 1 strain (LSCH-6B) was identified as Nocardiopsis sp. Of these, LS1-81 and LSCH-7 are possibly novel species of Streptomyces and Pseudonocardia respectively. These prospective novel actinomycete strains also exhibited significant antimicrobial activities. LS1-81 showedpotent activities against Micrococcus luteus, Bacillus subtilis and Aspergillus niger and LSCH-7 against Staphylococcus aureus, Micrococcus aureus, Bacillus subtilis and Aspergillus niger . This study highlights the promise of discovering novel bioactive actinomycetes in underexplored niche biotopes such as lake sediments.
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