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A NOVEL INTEGRON IN THE GENOME OF ESCHERICHIA COL I ISOLATED FROM INDONESIAN MONITOR LIZARD (VARANUS SPP).

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Author(s): STI PUSPIT ASAR | WALTER ERDEL EN | A NTONI US SUWANT O | AMAR ILA MA LIK

Journal: BIOTROPIA : the Southeast Asian Journal of Tropical Biology
ISSN 0215-6334

Volume: 16;
Start page: 10;
Date: 2001;
Original page

Keywords: Integron / antibiotic re si stance / Es cher ic hia coli

ABSTRACT
The ge no ty pe o f an ti bi ot ic re si sta nce in nat ural i solat es of Es ch er ichi a c oli was d ete rmin ed through integron detection and characterization of the associat ed antibiotic re sistance. E. coli SG2 is olated f rom Vara nus salvator of Java demonstrated re si stance to specti no myci n (5 0ng/ml) and st re pt omycin (SOng/ml). Inte gr on detection indi ca te d th at eight isol at es out of nine E. c oli isola tes possessed a conserved segment of the integron. Amplification of the inserted cassette of the integro n in this SG2 isolate y ielded a 1-kb DNA fragment. S equence analy ses indica ted t hat this fragment was homologous with aad ge ne, which confirm ed the re sistance to s pec tinomycin /stre ptom ycin. This i s t he first report on the presence of inte gron in t he E. coli isolated from the envi ronment.
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