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A Novel RP-HPLC method for simultaneous estimation of diacerhein and aceclofenac in oral pharmaceutical formulation.

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Author(s): Mohammad Zameeruddin*1, khurshid I. Molvi1, Sayyad Nazim1, Imran Khan2, Shaikh Siraj1, Afsar C. Shaikh1

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 8;
Start page: 2846;
Date: 2011;
Original page

Keywords: Aceclofenac | Diacerhein | RP-HPLC | Validation | Simultaneous estimation.

ABSTRACT
A selective and accurate high performance liquid chromatographic method has been developed and validated for simultaneous estimation of diacerhein (DIC) and aceclofenac (ACF) in oral pharmaceutical formulation. The chromatographic separation was achieved by RP- HPLC using mixture of methanol, water and acetic acid as mobile phase using HiQ Sil C18 column and UV detection at 275.0 nm. The method was validated for linearity, precision, accuracy, selectivity androbustness. All the parameters examined met the current recommendation of bioanalyical validation development. The proposed method can be successfully used for routine analysis, quality control and stability studies of marketed for oral pharmaceutical formulation.
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