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Nucleolar proteins change in altered gravity

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Author(s): Margarita A. Sobol | Fernando Gonzalez-Camacho | Elizabeth L. Kordyum | Francisco Javier Medina

Journal: Journal of Applied Biomedicine
ISSN 1214-021X

Volume: 5;
Issue: 1;
Start page: 49;
Date: 2007;
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Keywords: Clinorotation | Lepidium sativum | nucleolus | nucleolin-like protein | two-dimensional immunoblotting

ABSTRACT
Nucleolin is a major highly phosphorylated nucleolar protein involved in the regulation of r-chromatincondensation/expansion and rDNA transcription as well as in rRNA processing. The nucleolar proteinhomologous to the mammalian nucleolin and to the onion nucleolin-like protein NopA100, was detectedin the nuclear soluble protein fraction, and in the nuclear matrix fraction from Lepidium sativum rootmeristematic cells, using the selective silver staining method and the cross-reaction with the anti-NopA100 antibody. In 2-DE Western blots of both nuclear fractions, the nucleolin-like protein wasrevealed as a smear on the level of 90 kDa extending through a certain range of pI. In both extractsobtained from seedlings germinated and grown under slow clinorotation, the extension of the pI rangewas shorter and the molecular weight range was thinner than in the 1 g control; moreover, in the nuclearmatrix fraction, the spread of the pI range was separated into two clusters. The results obtained couldindicate a lower phosphorylation of the protein, suggesting a decrease in the activity of L. sativumnucleolin-like protein under clinorotation.
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