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Nycthemeral rhythms of total locomotor activity and oxidative markers in horse

Author(s): Giuseppe Piccione | Claudia Giannetto | Stefania Casella | Simona Marafioti | Vanessa Messina | Anna Assenza | Caterina Faggio | Francesco Fazio

Journal: Journal of Applied Biomedicine
ISSN 1214-021X

Volume: 9;
Issue: 1;
Start page: 43;
Date: 2011;
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Keywords: nycthemeral rhythm | free radicals | horse | oxidative power | locomotor activity

The aim of this study was to investigate the nycthemeral rhythm of total locomotor activity (TLA) in horseand the possible involvement of the daily organization of rest/activity cycles in the fluctuation of the redoxstate. For this purpose we recorded TLA and determined oxidative markers in ten clinically healthy ItalianSaddle horses. TLA was continuously recorded by means of an actigraphy-based data logger(Actiwatch-Mini®). For the assessment of free radicals (dROMs), the antioxidant barrier (Oxy-ads) and thethiol-antioxydant barrier (SHp), blood samples were collected every 4 hours over a 48 h period. One-wayrepeated measures analysis of variance (ANOVA) showed a statistically significant effect of time of day onall studied parameters. The application of the periodic model and the statistical analysis of cosinor indicate,in horses, the existence of a daily rhythm of the studied parameters during the 48 h of monitoring. The resultsshow that nycthemeral rhythms of TLA and oxidative markers have different trends in horse. dROMs andOxy-ads showed a nycthemeral rhythm with an acrophase in the middle of the photophase, and the acrophaseof SHp nycthemeral rhythm preceded them. In contrast, TLA showed its acrophase only after the middle ofthe photophase. TLA showed a lower robustness of rhythms (16.3 and 20.3%) and in respect to the robustnessvalues of the rhythms of oxidative markers (67.3–86.2%). In conclusion, the results of the presentinvestigation showed that oxidative markers have different patterns than locomotor activity, and furtherstudies could be necessary to determine whether other external stimuli, such as solar radiation, foodadministration or physical exercise are able to influence redox state rhythms in this species.
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