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Observation of Biochemical Imaging Changes in Human Pancreatic Cancer Tissue using Fourier-transform Infrared Microspectroscopy

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Author(s): Ying-Jen Chen | Yih-Dih Cheng | Hsin-Yi Liu, MS | Paul-Yann Lin | Chia-Siu Wang

Journal: Chang Gung Medical Journal
ISSN 2072-0939

Volume: 29;
Issue: 05;
Start page: 518;
Date: 2006;
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Keywords: pancreatic cancer | biochemical image | mapping | infrared microspectroscopy.

ABSTRACT
Fourier-transform infrared (FT-IR) microspectroscopic mapping can be used to distinguishbetween different tissue structures, and to increase the image contrast between normaland cancerous regions of a given tissue sample. This study demonstrates the biochemicalchanges associated with a consistent link between cancerous tissue and various molecularchanges in the IR spectra of human pancreatic cancer tissue using FT-IR mapping. Tissuesamples were obtained immediately after resection in a patient who underwent a distal pancreatectomyincluding the pancreatic body and tail. The biochemical imaging changes oflipids, proteins, and nucleic acids in human pancreatic cancer tissue were analyzed via FTIRmicrospectroscopy, using imaging, mapping, and line scan techniques. The intensitiesand frequencies of the absorption bands in the IR spectra of human pancreatic cancerous tissuewere markedly reduced and shifted, particularly in the amide bands of protein and CH2and CH3 stretching vibrations of lipids. The cancerous tissue contained significant proteincontent, and the distributions of DNA and lipid were very low, indicating low amounts ofnucleic acids and lipids in human pancreatic cancer tissue. The analytical results indicatethat these FT-IR microspectroscopic biochemical images reflect the distribution of cell components,which could be correlated with stained tissue in adenocarcinoma in pancreatic tissues.This study with samples of noncancerous and cancerous pancreatic tissues has clearlydemonstrated that FT-IR microspectroscopy using the mapping method can be used for diagnosis.
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