Academic Journals Database
Disseminating quality controlled scientific knowledge

Octreotide improves oxidative stress in sodium taurocholateinduced pancreatic injury

Author(s): Katerina Kotzampassi | D. Paramythiotis | Areti Makedou | E. Eleftheriadis

Journal: Annals of Gastroenterology
ISSN 1108-7471

Volume: 17;
Issue: 4;
Date: 2007;
Original page

Although the beneficial effects and some of the mechanism of action of octreotide in the treatment of acute pancreatitis are known, there is little information concerning its effect on the oxidative stress induced during the evolution of the disease. Therefore, we decided to investigate its possible action in a rat model of taurocholate-induced acute pancreatitis. Acute pancreatitis was induced in 16 male Wistar rats by injection of 0.5ml 5% sodium taurocholate into the duodenal loop, which remained closed for 60sec, while 16 more animals were sham operated. A dose of 4Ïg/kg octreotide or placebo (normal saline) was injected subcutaneously immediately after the procedure and 12hs later, thus creating 4 study-groups. At 24hs blood was obtained for the assessment of malondialdehyde (MDA) levels, as well as of the antioxidants enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx). The acute pancreatitis rats exhibited a statistically significant (p=0.01) elevation of MDA levels in relation to the sham operated or to sham operated-plus-octreotide groups. However, a highly significant decrease of SOD and GPx levels was prominent in acute pancreatitis group in relation to the sham operated or to sham operated-plus-octreotide groups. Octreotide treatment after acute pancreatitis induction resulted in a significant suppression of MDA levels and in a highly significant decrease of SOD and Gpx consumption versus the placebo treatment acute pancreatitis groups. The results obtained from this rat model indicate that the widely accepted beneficial effects of octreotide in the evolution of acute experimental pancreatitis could be partly explained by its ability to reduce oxidative stress. Key words: Octreotide, Experimental pancreatitis, Free radicals, Lipid peroxidation, Oxidative stress, Sodium taurocholate

Tango Rapperswil
Tango Rapperswil

     Save time & money - Smart Internet Solutions