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Optical Sum Frequency Generation Image of Rice Grains

Author(s): Hongyan Li | Yoshihiro Miyauchi | Nguyen Anh Tuan | Goro Mizutani | Mikio Koyano

Journal: Journal of Biomaterials and Nanobiotechnology
ISSN 2158-7027

Volume: 03;
Issue: 02;
Start page: 286;
Date: 2012;
Original page

Keywords: Optical Sum Frequency Generation Microscopy | Oryzaglutinosa (Rice) | Amylopectin | Starch | Crush Cell Layer | Endosperm

We observed optical sum frequency generation (SFG) images of cross-sections of glutinous rice grains, in order to test a possibility of the SFG microscopy as a tool for monitoring polysaccharide species in rice grains. The SFG response in the CH vibration range was the most intense in the crush cell layer at the edge of the endosperm adjacent to the embryo probably due to optical reflection and scattering effectby the rugged dielectric structure of the crush cell layer. The SFG spectra as a function of the infrared wavelength depended on the measurement position in the endosperm. The SFG results were compared with those by Raman and infrared spectroscopies for the same samples.
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