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OPTIMALISATION OF SPECIES IDENTIFICATION OF COMMON CARP (CYPRINUS CARPIO) USING SYBR® GREEN I REAL-TIME PCR METHOD

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Author(s): Pavol Bajzík | Radoslav Židek | Jozef Golian | Ľubomír Belej | Jozef Čapla | Lenka Maršálková | Ondrej Revák

Journal: Potravinarstvo : Scientific Journal for Food Industry
ISSN 1338-0230

Volume: 5;
Issue: 3;
Start page: 1;
Date: 2011;
Original page

Keywords: species identification | SYBR® Green I. Real-Time PCR | Cyprinus carpio

ABSTRACT
European Union Member States, together with a number of countries around the world, places great emphasis on ensuring the protection of consumers as a potential food allergic from food allergies. to inform consumers that their product may contain any of the risk of allergenic ingredients. For species identification of fish and fish products as a potential food allergens are used by many analytical methods as well as their authentication. We are in our work applied the method of SYBR® Green I. Real-Time PCR. We focused on pre-designed molecular - genetic marker of common carp (Cyprinus carpio), which comes from the mtDNA control D - loop area. We analyzed its presence in DNA isolates from the 5 kinds of freshwater fish, diluted to 10 % concentration. Results of using the optimized SYBR ® Green I Real-Time PCR method for species identification common carp (Cyprinus carpio) indicate to its suitability.doi:10.5219/154 
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